白介素8/IL-8蛋白/试剂盒，ACROBiosystems百普赛斯

IL-8产品信息

英文名称：Interleukin-8

中文名称：白细胞介素-8

靶点别称：CXCL8,GCP1,IL-8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1

物种：Human / Cynomolgus

属性：Protein / Kits

标记：Biotin-labeled / Unconjugated

IL-8 Protein

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

The sterility testing was performed by membrane filtration method.

支原体（Mycoplasma）

Negative.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

IL-8 ELISA Kit

背景（Background）

ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-8 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

应用说明（Application）

The kit is developed for quantitative detection of IL-8 in human serum and cell culture supernates.

It is for research use only.

原理（Assay Principles）

This assay kit is used to measure the concentrations of human Interleukin 8 (IL-8) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-8 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-8 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

IL-8分子背景

白细胞介素8（IL8或趋化因子（C-X-C基序）配体8，CXCL8）是由巨噬细胞和其他细胞类型（如上皮细胞、气道平滑肌细胞和内皮细胞）产生的趋化因子。表面膜上有许多能够结合IL-8的受体，最常被研究的类型是G蛋白偶联的蛇形受体CXCR1和CXCR2。两种受体对IL-8的表达和亲和力不同（CXCR1＞CXCR2）。与IL-8（1-77）相比，IL-8（6-77）对中性粒细胞活化的活性高出5-10倍。

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