Spike Protein ACROBiosystems百普赛斯

分子名称：Spike protein

英文名称：Coronavirus spike glycoprotein

中文名称：冠状病毒刺突糖蛋白

靶点别称：Spike,S protein,Spike glycoprotein,S glycoprotein

物种：人/鼠/猴等

属性：重组蛋白/抗体/试剂盒/磁珠/假病毒

标记：Biotin-labeled / Unconjugated

表达区间及表达系统（Source）

SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H9) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 ). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Predicted N-terminus: Val 16

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 138.0 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

据报道，SARS-CoV-2可通过与人ACE2受体的相互作用感染人类呼吸道上皮细胞。Spike protein是一种大的I型跨膜蛋白，包含两个亚基，S1和S2。S1主要包含一个受体结合结构域（RBD），负责识别细胞表面受体。S2包含膜融合所需的基本元素。S蛋白在诱导中和抗体和T细胞反应以及保护性免疫中起着关键作用。

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