产品名称：胶原蛋白酶 来源于溶组织梭菌(类型1)

规格或纯度：EnzymoPure™, ≥125 units/mg dry weight

产品介绍：

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.

Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities.

This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Accession #：P04180

级别：EnzymoPure™

浓度：≥125 units/mg dry weight

储存条件：2-8°C储存

运输条件：低温运输

CAS编号和信息：9001-12-1

酶学委员会编号：EC: 3.4.24.3

单位定义：One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.

Accession #：P04180

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