aladdin 阿拉丁 D292990 (2,3-二油酰基-丙基)-三甲胺（氯盐）DOTAP 132172-61-3 98%

中文名：(2,3-二油酰基-丙基)-三甲胺（氯盐）DOTAP

英文名：1,2-dioleoyl-3-trimethylammonium-propane (chloride salt) (DOTAP Chloride)

应用：1,2-dioleoyloxy-3-(trimethylammonium)propane (DOTAP) chloride has been used for the preparation of cationic liposome and liposome-DNA complex. It has also been used to assess tumor necrosis factor α (TNF- α) production. Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.

纯度：Moligand™，≥98%

货号：D292990

Cas号：132172-61-3

存储温度：避光,-20°C储存,充氩

运输条件：超低温运输

产品介绍：

1,2-二油氧基-3-(三甲铵)丙烷(DOTAP)氯化物已用于制备阳性脂质体和脂质体-DNA复合体。它还用于评估肿瘤坏死因子α (TNF- α)的产生。
由阳离子脂质体组成的化合物，用于向真核细胞中转染DNA、RNA及其他负电性分子。DNA与DOTAP混合时会自动形成稳定的复合物，后者可直接加入细胞培养基中。这些复合物能够与细胞膜融合并向细胞质中释放DNA。细胞被高效转染的同时不会产生细胞毒性。每μg DNA需使用5-10 μg左右的DOTAP，每mL 细胞培养基需加入约10-20 μg DOTAP。最佳的工作浓度和转染试剂视被转染的细胞系，以及转染试材类型（RNA、DNA、蛋白）而定。

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