产品名称：磷酸酶(碱性)来源于小牛肠(纯化)

规格或纯度：EnzymoPure™, ≥3,000 units/mg protein(37℃,pH9.8,DEA)

产品介绍：

The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sμgars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges. Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase.碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。

Accession #：P00634

级别：EnzymoPure™

浓度：≥3,000 units/mg protein(37℃,pH9.8,DEA)

储存温度：2-8°C储存

运输条件：低温运输

CAS编号和信息：9001-78-9

酶学委员会编号：3.1.3.1

单位定义：One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.

Accession #：P00634

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