Recombinant Alpha-Fetoprotein (AFP)

Recombinant Alpha-Fetoprotein (AFP)

Sequence Information

Species：Human Gene ID:174

Swiss Prot:P02771 Synonyms:aFP; A-FP; FETA; HPAFP;

Alpha-Fetoglobulin; Alpha-1-fetoprotein

Residues:Ile31~Ser576

CTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFS
Product Information

Source: Prokaryotic expression. Host: E. coli

Tags: Two N-terminal Tags, His-tag and SUMO-tag

Subcellular Location: Secreted. Purity: >90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl,5%Trehalose.
Original Concentration: 50µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 75.2kDa

Accurate Molecular Mass: 75kDa as determined by SDS-PAGE reducing conditions.

[USAGE ]

Reconstitute in ddH₂Oto a concentration of 0.1-0.5 mg/mL. Do not vortex. 

[STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. 
Store at 2-8℃ for one month.
Aliquot and store at -80℃ for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37℃for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 5% within the expiration date under appropriate storage condition.

[IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for in vitro and research use only, we will not be responsible for anyissue if the kit was used in clinical diagnostic or any other procedures.

仅供科研使用。不能用于人和动物治疗等其它临床诊断用！