phospho-IRE1 (Ser724) 磷酸化IRE1蛋白抗体

Rabbit  Anti-phospho-IRE1 (Ser724) Polyclonal Antibody
DATASHEET

Host:Rabbit

Target Protein:phospho-IRE1 (Ser724)

IR:Immunogen Range:RH(p-S)FS

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2081

Swiss Prot:O75460

Source:KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724:RH(p-S)FS 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway

Size:50ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Sheep

本品仅供科研使用。不能用于人和动物治疗等其它临床诊断用