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This instruction of tetrodotoxin determination in aquatic product focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with LC-MS. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed. Many pretreatment methods of This instruction of tetrodotoxin determination in aquatic product focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with LC-MS. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed. Many pretreatment methods of tetrodotoxin determination in food and feed show low sensitivity because of interfering substances if problematic matrices are applied. This method of content determination of tetrodotoxin combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate eluate and additional step of purification by LC-MS column.

Tetrodotoxin determination in aquatic product show low sensitivity because of interfering substances if problematic matrices are applied. This method of content determination of tetrodotoxin combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate eluate and additional step of

purification by LC-MS column.