产品详情
纯度：
Greater than 93% as determined by SDS-PAGE.
内毒素：
Less than 1.0 EU/ug as determined by LAL method.
生物活性：
①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody(CSB-RA156386A0HU), the EC50 is 2.353-3.232 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized CD40(CSB-MP004936HU1) at 2 μg/ml can bind CD40L, the EC50 is 3.112-3.858 ng/ml. ③Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay.
基因名：
CD40LG
Uniprot No.：
P29965
别名：
(CD40-L)(T-cell antigen Gp39)(NF-related activation protein)(TRAP)(Tumor necrosis factor ligand superfamily member 5)(CD154)(sCD40L)
分子结构：
种属：
Homo sapiens (Human)
蛋白长度：
Partial
来源：
Mammalian cell
分子量：
44.5 kDa
表达区域：
113-261aa
氨基酸序列
MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
蛋白标签：
N-terminal hFc-Flag-tagged
产品提供形式：
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
复溶：
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：
3-7 business days
注意事项：
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
产品描述：
Human CD40 ligand (CD40LG) amino acid Met13-Leu261, with an N-terminal hFc-Flag-tag as well as an N-terminal linker, was expressed in mammalian cells. The product is the recombinant human CD40LG protein. The SDS-PAGE analysis determined its purity greater than 90% and showed a molecular weight band of about 45 kDa on the gel. It contains low levels of endotoxin, less than 1.0 EU/ug measured by the LAL method. Functional ELISA and LSPR assay of this CD40LG protein showed that it is an active protein. This protein is in stock so that it will reach your lab bench faster.
CD40LG, also known as CD154, is a ligand for the CD40. It is preferentially expressed by activated CD4+ T cells as well as activated B cells and platelets. The interaction of CD40 and CD40LG plays an essential role in immune regulation and homeostasis. The CD40-CD40L system is also involved in the pathophysiology of several diseases, including autoimmune diseases, atherothrombosis, respiratory disease, and cancer.
艾普蒂生物科技（ipodix）多年来致力于重组蛋白表达与纯化的相关研究，每年可完成上百组蛋白的表达与纯化服务，拥有完整的蛋白纯化体系，可根据客户的不同需求提供多元化的蛋白纯化方案。我们可为您提供定制的蛋白纯化服务包括，有/无标签重组蛋白，天然蛋白的纯化分离等。根据蛋白性质设计亲和纯化、离子交换、分子筛、疏水等多种蛋白纯化实验方案，获得的高纯度蛋白质，可根据客户需求一对一进行个性化方案定制，能够满足蛋白质折叠、重结晶等一系列精细实验要求。
重组蛋白纯化：
重组蛋白分离纯化一般分为三种：含标签的重组蛋白纯化、不含标签的重组蛋白纯化以及包涵体变性与复性纯化。
含标签的重组蛋白纯化：组氨酸标签（His-tag）是使用范围较为广泛的一种蛋白纯化标签，其纯化的原理是组氨酸残基上的咪唑基团可与层析介质中的金属离子螯合，再通过提高缓冲液中咪唑浓度进行竞争性洗脱达到纯化效果，这种纯化标签的有点在于His-Tag的分子质量非常小且几乎不会对目的蛋白本身的生物学功能及特性产生影响，因此带有His-Tag的蛋白纯化后可直接作为抗原免疫动物制备抗体并不会影响抗体的特异性。艾普蒂生物还提供其他多种类型标签的蛋白纯化服务，如GST-Tag、SUMO-Tag、MBP-Tag等。
图1 6xHis标签和Ni-NTA相邻残基之间的相互作用
不含标签的重组蛋白纯化：不含纯化标签的重组蛋白我们会根据客户的样品及需求定制方案，一般会采用抗体亲和纯化方法，利用靶向配体能与目的蛋白特异性结合的特性将目的蛋白分离出来；或选用分子筛进行分离纯化。
包涵体变性与复性纯化：将以包涵体形式存在的蛋白进行变复性操作，根据蛋白质不同的性质，可以选择尿素、盐酸胍、去垢剂、极端pH等，以保证程度获得单体状态的变性蛋白。
艾普蒂生物提供的蛋白分离纯化流程：
步骤 | 服务内容 | 周期 |
Step1 纯化前检测与预实验 | SDS-PAGE检测蛋白情况； 小体积纯化与检测 | 1-2周 |
Step2 蛋白纯化及检测 | 大量纯化； SDS-PAGE； WB检测（视项目而定）； 浓度检测 | 1-2周 |
交付 | 成品蛋白，纯化报告，图片数据； |
服务优势：
--设备一流，纯化方式多元化，适合不同性质的重组蛋白；
--专业技术团队为您量身定制纯化方案，获得能够满⾜下游实验的⾼纯度蛋⽩；
--提供全面的蛋白纯化分析报告；
