产品详情
产品描述:
This Human BMP4 ELISA Kit was designed for the quantitative measurement of Human BMP4 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名:
zgc:100779 ELISA Kit; BMP 2B ELISA Kit; BMP 4 ELISA Kit; BMP-2B ELISA Kit; BMP-4 ELISA Kit; BMP2B ELISA Kit; BMP2B1 ELISA Kit; BMP4 ELISA Kit; BMP4_HUMAN ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; Bone morphogenetic protein 4 ELISA Kit; DVR4 ELISA Kit; MCOPS6 ELISA Kit; MGC100779 ELISA Kit; OFC11 ELISA Kit; zbmp-4 ELISA Kit; ZYME ELISA Kit
缩写:BMP4
种属:
Homo sapiens (Human)
样本类型:
serum, plasma, cell culture supernates
检测范围:
15.6 pg/mL-1000 pg/mL
灵敏度:
3.9 pg/mL
反应时间:
1-5h
样本体积:
50-100ul
检测波长:
450 nm
研究领域:
Signal Transduction
测定原理:
quantitative
测定方法:
Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human BMP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
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1:1 | Average % | 94 |
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Range % | 89-97 |
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1:2 | Average % | 103 |
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Range % | 95-107 |
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1:4 | Average % | 92 |
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Range % | 82-94 |
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1:8 | Average % | 95 |
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Range % | 89-99 |
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回收率:
The recovery of human BMP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range |
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Serum (n=5) | 94 | 90-98 |
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EDTA plasma (n=4) | 86 |
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注意事项:
1、试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存
2、浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果
3、各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样
4、请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定计算时请最后乘以总稀释倍数(xnx5)
5、封板膜只限一次性使用,以避免交叉污染
6、底物请避光保存。
7、严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准
8、所有样品,洗涤液和各种废弃物都应按传染物处理
9、.本试剂不同批号组分不得混用。
艾普蒂生物 品牌保障:
1、质量保证: 产品质量稳定,每批次产品必须通过QA、QC检测才可出库;
2、技术支持: 技术部5x8小时技术问题及时解答
3、服务周到: 发货及时,三个工作日顺丰快递
4、售后无忧:产品质量问题包退包换
艾普蒂ELISA试剂盒的优势:
1、高效、灵敏、特异的抗体;
2、稳定的重复性和可靠性;
3、吸附性能好,空白值低,孔底透明度高的固相载体;
4、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型!