人骨成型蛋白2(BMP-2)Elisa试剂盒

产品详情

• 产品描述：

  This Human BMP2 ELISA Kit was designed for the quantitative measurement of Human BMP2 protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

• 别名：BDA2 ELISA Kit; BMP-2 ELISA Kit; BMP-2A ELISA Kit; Bmp2 ELISA Kit; BMP2_HUMAN ELISA Kit; BMP2A ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit

• 缩写：BMP2

• Uniprot No.：

• 种属：Homo sapiens (Human)

• 样本类型：serum, urine, cell culture supernates, tissue homogenates

• 检测范围：62.5 pg/mL-4000 pg/mL

• 灵敏度：15.6 pg/mL

• 反应时间：1-5h

• 样本体积：50-100ul

• 检测波长：450 nm

• 研究领域：Signal Transduction
  

• 测定原理：quantitative

• 测定方法：Sandwich

• 精密度：

  Intra-assay Precision (Precision within an assay): CV%<8%
  Three samples of known concentration were tested twenty times on one plate to assess.
  Inter-assay Precision (Precision between assays): CV%<10%
  Three samples of known concentration were tested in twenty assays to assess.

• 线性度：

  To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  		Sample		Serum(n=4)
  1:1		Average %		90
  		Range %		80-95
  1:2		Average %		95
  		Range %		91-99
  1:4		Average %		97
  		Range %		92-106
  1:8		Average %		93
  		Range %		86-99

  回收率：

  The recovery of human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
  Sample Type		Average % Recovery		Range
  Serum (n=5)		95		89-100

1、试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存

2、浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果

3、各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样

4、请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值)，请先用样品稀释液稀释一定倍数(n倍)后再测定计算时请最后乘以总稀释倍数(xnx5)

5、封板膜只限一次性使用，以避免交叉污染

6、底物请避光保存。

7、严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准

8、所有样品，洗涤液和各种废弃物都应按传染物处理

9、.本试剂不同批号组分不得混用。

艾普蒂生物 品牌保障：

1、质量保证: 产品质量稳定，每批次产品必须通过QA、QC检测才可出库;

2、技术支持: 技术部5x8小时技术问题及时解答

3、服务周到: 发货及时，三个工作日顺丰快递

4、售后无忧:产品质量问题包退包换

艾普蒂ELISA试剂盒的优势:

1、高效、灵敏、特异的抗体;

2、稳定的重复性和可靠性;

3、吸附性能好，空白值低，孔底透明度高的固相载体;

4、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型!