别名：ApoL related ligand TALL 1 ELISA Kit; B cell Activating Factor ELISA Kit; B lymphocyte stimulator ELISA Kit; B-cell-activating factor ELISA Kit; BAFF ELISA Kit; BLyS ELISA Kit; CD 257 ELISA Kit; CD257 ELISA Kit; CD257 antigen ELISA Kit; Delta BAFF ELISA Kit; Dendritic cell derived TNF like molecule ELISA Kit; Dendritic cell-derived TNF-like molecule ELISA Kit; DTL ELISA Kit; DTL precursor ELISA Kit; PRO738 ELISA Kit; soluble form ELISA Kit; TALL 1 ELISA Kit; TALL-1 ELISA Kit; TALL1 ELISA Kit; THANK ELISA Kit; TN13B_HUMAN ELISA Kit; TNF and APOL related leukocyte expressed ligand 1 ELISA Kit; TNF homolog that activates apoptosis ELISA Kit; TNF homolog that activates apoptosis NKFB and JNK. ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit; TNFSF13B ELISA Kit; TNFSF20 ELISA Kit; TNLG7A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 13b ELISA Kit; Tumor necrosis factor ligand 7A ELISA Kit; Tumor necrosis factor ligand superfamily member 13b ELISA Kit; Tumor necrosis factor ligand superfamily member 20 ELISA Kit; Tumor necrosis factor like protein ZTNF4 ELISA Kit; Tumor necrosis factor superfamily member 13B ELISA Kit; UNQ401 ELISA Kit; ZTNF4 ELISA Kit
缩写：TNFSF13B
Uniprot No.：Q9Y275
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度： 0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human BAFF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 88
Range % 82-95
1:2 Average % 90
Range % 85-96
1:4 Average % 93
Range % 90-95
1:8 Average % 92
Range % 88-96
回收率：
The recovery of human BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 85-99
EDTA plasma (n=4) 90 87-94
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.912 2.953 2.933 2.819
10 2.142 2.175 2.159 2.045
5 1.295 1.312 1.304 1.190
2.5 0.727 0.695 0.711 0.597
1.25 0.426 0.443 0.435 0.321
0.625 0.297 0.312 0.305 0.191
0.312 0.247 0.261 0.254 0.140
0 0.112 0.115 0.114
注意事项：
1、试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存
2、浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果
3、各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样
4、请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值)，请先用样品稀释液稀释一定倍数(n倍)后再测定计算时请最后乘以总稀释倍数(xnx5)
5、封板膜只限一次性使用，以避免交叉污染
6、底物请避光保存。
7、严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准
8、所有样品，洗涤液和各种废弃物都应按传染物处理
9、.本试剂不同批号组分不得混用。
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