LightOx<SUP>?</SUP>14,198696-03-6

应用

Fluorescence microscopy, fluorescent retinoid binding assay, fluorescence spectroscopy.

特点和优势

LightOx14 is a synthetic analogue of all-trans-retinoic acid (ATRA) that exhibits solvatochromatic fluorescence emission when excited with UV or 405 nm light. The compound is a strong ligand for the retinoic acid receptor proteins (RARs) and related cellular retinoic acid binding proteins (CRABPI/II) and can, therefore, be used as a replacement for ATRA in cellular assays involving retinoid signaling processes. LightOx14 can be utilized to study retinoid behaviour using fluorescence microscopy, employed in a competitive fluorometric ligand-binding assay for direct determination of the strength of binding of non-fluorescent retinoids to a protein target, and as a fluorophore in fluorescence spectroscopy.