Anti-DNA:RNA hybrid抗体[clone:S9.6]

Description

DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.

Catalog No.

RGK60001

Host species

mouse

Tested applications

CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IP, SPR, IF

Species reactivity

General

Form

liquid

Storage buffer

0.01M PBS, pH 7.4.

Concentration

0.74 mg/ml

Purity

>95%

Clonality

Monoclonal

Isotype

IgG2a, kappa

Applications

CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IP, SPR, IF

Purification

Protein A or G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Expression system

Mammalian cells

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.

Reconstitution

Reconstitute in sterile water for a stock solution.

Alternative Names

DNA-RNA Hybrid

Note

For research use only.

Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170

Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514

High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798

RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442

The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954

Structural basis of R-loop recognition by the S9.6 monoclonal antibody. PMID: 35347133

Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340

Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287

The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567

The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6. PMID: 23784994

Modifications and interactions at the R-loop. PMID: 32961406

BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180

R-Loop Analysis by Dot-Blot. PMID: 33554969

Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345

New insight into the biology of R-loops. PMID: 32516653

Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452

Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733

Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. PMID: 25890199

Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394