L-Leucine 4-methoxy-β-naphthylamide

产品属性：

Cas No.4467-68-9

别名 Leu-MNA

化学名 (S)-2-amino-N-(4-methoxy-2-naphthalenyl)-4-methyl-pentanamide, monohydrochloride

分子式 C17H22N2O2 ? HCl

分子量 322.8

溶解度 ≤25mg/ml in ethanol;10mg/ml in DMSO;20mg/ml in dimethyl formamide

储存条件 Store at -20°C
General tips  For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition     Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述：
L-Leucine 4-methoxy-β-naphthylamide is a substrate for aminopeptidase M and leucine aminopeptidase.

Aminopeptidases are enzymes catalyzing the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides. Aminopeptidases are distributed widely throughout the animal and plant kingdoms and are present in various subcellular organelles, in cytosol, as well as membrane components.

In vitro: L-Leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities [1]. In a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including L-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. Selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. This method was successfully validated by measurements in cells isolated from cathepsin B-/--, K-/--, and L-/-- mice [2].

In vivo: Up to now, there is no animal in vivo data reported.

Clinical trial: So far, no clinical study has been conducted.

References:

[1] Monis, B. ,Wasserkrug, H. and Seligman, A.M. Comparison of fixatives and substrates for aminopeptidase. Journal of Histochemistry and Cytochemistry 13(6), 503-509 (1965).

[2] Rüttger, A. ,Mollenhauer, J.,Lser, R., et al. Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. Biotechniques 41(4), 469-473 (2006).