基本信息 产品详情 公司简介 推荐产品
网站主页 BRAF(V600E) 小鼠单抗 BRAF(V600E) 小鼠单抗
  • BRAF(V600E) 小鼠单抗

BRAF(V600E) 小鼠单抗

Anti-BRaf(V600E) Mouse Monoclonal Antibody
4800 100微升 起订
湖北 更新日期:2023-04-16

武汉费斯德生物科技有限公司

非会员
联系人:黎敏
电话:027-63497286拨打
手机:18162422833 拨打
邮箱:nebio0923@163.com

产品详情:

中文名称:
BRAF(V600E) 小鼠单抗
英文名称:
Anti-BRaf(V600E) Mouse Monoclonal Antibody
品牌:
NewEastbio
保存条件:
-20
产品类别:
Oncogenes点突变抗体
货号:
26039
货号:   26039

基因符号:   BRaf

点突变.jpg

描述:   Anti-BRaf(V600E) Mouse Monoclonal Antibody
背景介绍:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers
免疫原:   A synthetic peptide from the internal region of BRaf(V600E), human origin
经过测试的应用:   ELISA, WB, IHC, IP
推荐稀释比:   
ELISA: 1: 100-1: 5000
WB: 1: 100-1: 2000
IHC: 1: 100-1: 1000
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG2b
纯度:   Purified from ascites
防腐剂:   No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing


Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.

Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .

免疫荧光:   

Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).

Immunohistochemistry:   

B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.

310936477332.png


Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.

图片.png

Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.Allele specific PCR validated to be Negative for BRAF V600E.



BRaf(V600E)抗体;BRAF(V600E) 小鼠单抗;

公司简介

武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处,也是目前国内唯一经销处。 目前客户使用产品发表超过1000谷歌学术文献,其中论文分布排名前50刊物上。 NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。 更多产品类目和技术资料可查看:武汉费斯德生物官网。 武汉费斯德生物科技有限公司将向你提供以下,抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。 注:2021年武汉费斯德生物科技有限公司和NewEast Biosciences收购了武汉纽斯特生物技术有限公司所有的产品和细胞株。因此, 纽斯特不再拥有这些产品销售权。如果您在 2022 年 6 月 1 日之后从 纽斯特购买相同的产品, 你不应该在你的论文中引用 纽斯特的产品为NewEast Biosciences产品。 否则,NewEast Biosciences 会去找出版商对你的引用提出异议。请注意, 用国际供应商的产品将会增加您发表文章citation的数量。 选择正品费斯德经销处,为您实验和相关文章提供保障,售后服务到位,产品现货供应。 ** 本公司销售的所有产品仅供实验科研使用,不用于人体及临床诊断。

成立日期 (3年)
注册资本 100万人民币
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 工厂,试剂,定制,服务
主营行业 抗体,细胞培养,癌症研究,细胞生物学,免疫安全

BRAF(V600E) 小鼠单抗相关厂家报价

内容声明
拨打电话 立即询价