重组人谷胱甘肽S-转移酶Pi1

中文名称

【GSTP1】重组人谷胱甘肽S-转移酶Pi1

英文名字

Recombinant Human Glutathione S-Transferase pi 1

供应商

ProSpec

产品货号

ENZ-427

背景资料

GSTP1 is a polymorphic gene encoding active， functionally different GSTP1 variant proteins that are believed to function in xenobiotic metabolism and have a part in susceptibility to cancer， and other diseases. GSTP1 is a glutathione S-transferase belonging to the pi class. GST family enzymes play a significant role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based upon the biochemical， immunologic and structural properties of the soluble GSTs they are grouped into 4 main classes: alpha， mu， pi， and theta. The GSTP1 enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a Sulfur-substituted glutathione. The reactions employing glutathione contribute the transformation of a broad range of electrophiles， including reactive products of lipid， protein， carcinogens， therapeutic drugs， environmental toxins， and products of oxidative stress.
The GSTP1 inactivation through CpG hypermethylation is frequent in pituitary adenomas and may be a factor in aggressive pituitary tumor behavior. GSTP1 is may be a transcriptional target of the p53 tumor suppressor gene. Single-nucleotide polymorphism in GSTP1 is linked to modified protein binding， which influence GSTP1’s contribution to carcinogen and drug metabolism， and possibly disease pathogenesis and/or drug response. GST-pi might have central roles in proliferation of androgen-independent human prostate cancer cells.

序列

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ.

来源宿主

大肠杆菌中重组表达

溶解建议

提供的人谷胱甘肽S-转移酶Pi1（GSTP1）产品为过滤灭菌的无色溶液；其中人谷胱甘肽S-转移酶Pi1（GSTP1）产品溶解于20mM Tris-HCl缓冲液（pH 7.0)，30%甘油，1mM EDTA，0.1mM PMSF溶液中.

保存建议

人谷胱甘肽S-转移酶Pi1（GSTP1）溶液可以在4℃冷藏保存4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存.请避免反复冻融.

其他

ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。 领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。