中文名称

细胞渗透性 Rho 抑制剂（基于C3转移酶）

英文名字

Cell Permeable Rho Inhibitor (C3 Trans based))

供应商

Cytoskeleton

产品货号

CT04-A

背景资料

该细胞渗透性 Rho 抑制剂（基于C3转移酶）产品是Cytoskeleton品牌原装进口，Cytoskeleton品牌是细胞骨架研究专家，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。 

产品描述

This product consists of highly purified C3 Transferase (Cat. # CT03) covalently linked to a proprietary cell penetrating moiety via a disulfide bond.  The cell penetrating moiety allows rapid and efficient transport through the plasma membrane.  Once in the cytosol, the cell penetrating moiety is released, thereby allowing C3 Transferase to freely diffuse intracellularly and inactive RhoA, RhoB, and RhoC, but not related GTPases such as Cdc42 or Rac1.The Exoenzyme C3 Transferase from Clostridium botulinum is commonly used to selectively inactivate the GTPases RhoA, RhoB, and RhoC, both in vivo and in vitro.  C3 Transferase inhibits Rho proteins by ADP-ribosylation on asparagine 41 in the effector binding domain of the GTPase.  A major limitation of C3 Transferase for use in in vivoapplications is that this protein is only slightly cell permeable.  Consequently, overnight incubations with C3 Transferase at concentrations as high as 100 µg/ml are often necessary to inactivate Rho proteins in cultured cells.  Under these conditions, the long incubation period and large amount of C3 Transferase that are required can be disruptive to basic cellular functions and economically burdensome.  Cytoskeleton, Inc. has overcome these problems by providing a cell permeable form or C3 Transferase that can efficiently inactivate cellular Rho proteins in as little as 2 h. 

产品形式

该细胞渗透性 Rho 抑制剂（基于C3转移酶）产品是Cytoskeleton品牌的原装进口产品，具体的产品形式查看英文产品说明书。

保存建议

厂家推荐常温运输，收到产品后建议保存于4°C。

其他

Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。