中文名称

Rho/Rac/Cdc42 激活剂 I

英文名字

Rho/Rac/Cdc42 Activator I

供应商

Cytoskeleton

产品货号

CN04-A

背景资料

该Rho/Rac/Cdc42 激活剂 I产品是Cytoskeleton品牌原装进口，Cytoskeleton品牌是细胞骨架研究专家，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。 

产品描述

The G-switch™ Direct line of small G-protein modulators have been developed with an emphasis on creating highly potent cell permeable reagents that directly target the Rho family of small G-proteins.  Our Direct Activator reagents are based on the catalytic domain of the bacterial CNF toxins, which are covalently attached to a proprietary cell penetrating moiety.  The Rho/Rac/Cdc42 Activator I (Cat# CN04) enters the cell and activates Rho GTPase isoforms by deamidating glutamine-63 of Rho and glutamine-61 of Rac and Cdc42 in their respective Switch II regions (1,2).  This modification converts glutamine-63 to glutamate, which blocks intrinsic and GAP stimulated GTPase activity resulting in constitutively active Rho (3).  The Rho/Rac/Cdc42 Direct Activator robustly increases the level of GTP bound RhoA, Rac1 and Cdc42 within 2-4 h after addition to the culture medium and thus provides a more rapid alternative to transfection based methods for introducing activators like GEFs into cells.  Moreover, the targeted action of the Rho Direct Activator makes it far more attractive tool for the study of Rho GTPase signaling than classic indirect activators (e.g. LPA, EGF, Bradykinin and Sphingosine-1-phosphate) that concomitantly activate other signaling pathways (e.g. Ras, PI3K and PLC).

产品形式

该Rho/Rac/Cdc42 激活剂 I产品是Cytoskeleton品牌的原装进口产品，具体的产品形式查看英文产品说明书。

保存建议

厂家推荐常温运输，收到产品后建议保存于4°C。

其他

Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。