尿液无细胞循环DNA纯化大提试剂盒（提取试剂盒）

中文名称

尿液无细胞循环DNA纯化大提试剂盒（提取试剂盒）

英文名字

Urine Cell-Free Circulating DNA Purification Maxi Kit

供应商

Norgen Biotek

产品货号

NGB-56800

背景资料

Recent evidence indicates that cell-free circulating DNA (cf-DNA) contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood). Urine cell-free circulating DNA (cf-DNA) has been utilized for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases, as well as for investigating fetal DNA that is normally present in the maternal blood. This cf-DNA is usually present as short fragments, generally between 150 and 250 bp or its duplicates, and is derived either from the apoptotic process, necrotic process or from the fetus.

产品描述

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples from volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. For a schematic workflow of the protocol click here. The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.

产品特点

Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples; Isolate viral DNA; Versatile urine input volumes (10 mL - 30 mL); Concentrate circulating DNA into a flexible elution volume ranging from (50 µL - 100 µL); Isolate inhibitor-free cell-free circulating DNA; Purify high-quality DNA in 40-45 minutes; Compatible with Norgen’s Urine Preservative and other commercially available urine preservatives.

保存建议

溶液密封室温(15-25°C)保存，未开封至少保存2年。具体保存情况收到产品后请按说明书存放。

其他

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。 目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。