血浆/血清循环和核外RNA纯化试剂盒（提取试剂盒）

中文名称

血浆/血清循环和核外RNA纯化试剂盒（提取试剂盒）

英文名字

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) (50)

供应商

Norgen Biotek

产品货号

NGB-42800

背景资料

血浆/血清循环和核外RNA纯化试剂盒（提取试剂盒）产品的背景资料详情查看Norgen Biotek品牌提供的产品说明书

产品描述

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL. This kit is able to isolate all sizes of circulating and exosmal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.Preparation time for a single sample is less than 40 minutes. The purified plasma/serum free-circulating and exosmal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

产品特点

Isolate all sizes of circulating and exosomal RNA, including microRNA; Versatile plasma and serum input volumes (0.25 mL to 5 mL); Concentrate circulating and exosomal RNA into small elution volumes; Isolate inhibitor-free circulating RNA; Bind and elute all RNA irrespective of size or GC content, without bias.

保存建议

所有缓冲液应密封保存，并在室温（15-25°C）下保存长达1年。具体保存情况收到产品后请按说明书存放。

其他

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。 目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。