Arigo 激活用 HMGB1 活性蛋白，ARG70220

Human HMGB1 recombinant protein (Active) (His-tagged, C-ter)

询价 20μg 起订

广东更新日期：2022-09-16

广州安邦生物科技有限公司

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产品详情:

中文名称：: Arigo 激活用 HMGB1 活性蛋白，ARG70220

英文名称：: Human HMGB1 recombinant protein (Active) (His-tagged, C-ter)

保存条件：: -20°C

产品类别：: 生物试剂

ARG70220

Human HMGB1 recombinant protein (Active) (His-tagged, C-ter)

Datasheet

产品说明书

概述

产品描述	E. coli expressed, His-tagged (C-ter) Active Human HMGB1 recombinant protein.
反应物种	Hu, Ms
应用	FuncSt, SDS-PAGE
靶点名称	HMGB1
物种	Human
A.A. 序列	MGKGD PKKPR GKMSS YAFFV QTCRE EHKKK HPDAS VNFSE FSKKC SERWK TMSAK EKGKF EDMAK ADKAR YEREM KTYIP PKGET KKKFK DPNAP KRPPS AFFLF CSEYR PKIKG EHPGL SIGDV AKKLG EMWNN TAADD KQPYE KKAAK LKEKY EKDIA AYRAK GKPDA AKKGV VKAEK SKKKK EEEED EEDEE DEEEE EDEED EDEEE DDDDE with polyhistidine tag at the C-terminus.
表达系统	E. coli
活动	Active
活动说明	Measure by its ability to induce TNF alpha in Raw264.7 cells. The ED50 for this effect is < 10 μg/ml. This protein also induces TNF alpha releasing from human A549 cultured cells based on customer feedback.
別名	HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

属性

形式	Powder
纯化	Ni-NTA chromatography.
纯化说明	Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
纯度	> 98% (by SDS-PAGE)
缓冲液	PBS (pH 8.0)
重建	It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放说明	Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 15289 Mouse HMGB1 GeneID: 3146 Human HMGB1 Swiss-port # P09429 Human High mobility group protein B1 Swiss-port # P63158 Mouse High mobility group protein B1
基因名称	HMGB1
全名	high mobility group box 1
背景介绍	HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
生物功能	HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
细胞定位	Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. Note=In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus. Translocates from the nucleus to the cytoplasm upon autophagy stimulation. Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes. [UniProt]
产品亮点	Related products: HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Total solution for HMGB1 research HMGB1 in inflammation Inflammatory Cytokines HMGB1 ELISA Kit for your research Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit New HMGB1 neutralizing antibody is released Detecting exosomal HMGB1 for ICD research Related poster download: HMGB1 Pathway.pdf
预测分子量	25 kDa
翻译后修饰	Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460). Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity). Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so). Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity). In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity). [UniProt]

活性蛋白;HMGB1;

安邦生物——专业提供高品质的实验室产品与服务，让生命科学研究更简单！广州安邦生物科技有限公司（Guangzhou abbiotechnology, Inc.）成立于2011年6月，坐落于广州市天河区怡祥大地公园，拥有400多平米的现货试剂耗材及仪器仓库，拥有专业的试剂冷库及配套冷链物流配送服务，是一家华南地区专业提供生命科学实验室产品和服务的供应商。公司主营业务涉及生物技术、生物医药、分子诊断、分析检测等领域，现阶段产品涵盖了细胞生物学、免疫学、分子生物学、表观遗传学、蛋白纯化、生化试剂等，产品线日趋完善。安邦生物核心团队皆是从事生物技术行业多年的资深人士，专业为客户提供个性化和定制化的一站式科研实验室产品解决方案，以及稳定可靠的技术支持与售后服务。

成立日期	(14年)
注册资本	800万人民币
员工人数	10-50人
年营业额	￥ 300万-500万
经营模式	贸易,试剂,服务
主营行业	化学试剂,细胞培养,蛋白组学,分子生物学