IRDye 700 AP-1 Consensus Oligonucleotide共有寡核苷酸

IRDye 700 AP-1 Consensus Oligonucleotide

IRDye 700 CREB Consensus Oligonucleotide

IRDye 700 HIF-1 Consensus Oligonucleotide

IRDye 700 p53 Consensus Oligonucleotide

IRDye 700 STAT3 Consensus Oligonucleotide

IRDye 700 Sp-1 Consensus Oligonucleotide

IRDye 700 NFĸB Consensus Oligonucleotide

IRDye 700 AP-1 共有寡核苷酸

5' -- CGC TTG ATG ACT CAG CCG GAA -- 3'

3' -- GCG AAC TAC TGA GTC GGC CTT -- 5'

带下划线的核苷酸是结合位点。 IRDye 700 寡核苷酸以 25 µL 的 50 nM（或 50 fmol/µL）双链 DNA 形式提供。

AP-1 是一种转录因子，可调节基因表达并控制许多细胞过程，包括分化、增殖和凋亡。 出于这个原因，它是 EMSA 中用于评估蛋白质结合的常见寡核苷酸。

由于 IRDye 红外染料对光有些敏感，因此在孵育期间将结合反应保持在黑暗中（例如，将试管放入抽屉或简单地用铝箔盖住装有试管的架子）。 孵育期后，将 10X Orange Loading Dye (P/N 927-10100) 添加到结合反应中进行电泳。

5′ -- CGC TTG ATG ACT CAG CCG GAA -- 3′

3′ -- GCG AAC TAC TGA GTC GGC CTT -- 5′

Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

AP-1 is a transcription factor that regulates gene expression and controls a number of cellular processes including differentiation, proliferation, and apoptosis. For this reason, it is a common oligo used in EMSA to assess protein binding.

Since IRDye infrared dyes are somewhat sensitive to light, it is best to keep binding reactions in the dark during incubation periods (e.g., put the tubes into a drawer or simply cover the rack containing tubes with aluminum foil). After the incubation period, 10X Orange Loading Dye (P/N 927-10100) is added to the binding reaction for electrophoresis.