IRDye 650 NHS Ester/Maleimide/Carboxylic acid

IRDye 650 NHS Ester

IR 650 NHS酯（IRDye® 650 NHS酯类似物）是一种水溶性，明亮，远红荧光染料，光谱上类似于用于流式细胞术，显微镜和其他应用的其他650染料。IR 650 共轭物适合 633 nm 或 647 nm 激光线。与其他荧光团相比，使用长波长染料（如Cy5®染料或Alexa Fluor® 647染料）的优势是生物标本在该光谱区域中的自发荧光较低。

NHS酯（或琥珀酰亚胺酯）是**常用的胺反应基团，用于标记蛋白质伯胺（Lys），胺修饰寡核苷酸和其他含胺分子。蛋白质可以用IR 650 NHS酯以高摩尔比标记，而不会显着的自熄灭，从而导致更亮的偶联物和更灵敏的检测。IR 650偶联物可用于检测低丰度靶标。

　　ReactivityPrimary amine

　　Abs/Em Maxima651/668 nm

　　Extinction coefficient230,000 cm-1M-1

　　SolubilityWater, DMSO, DMF

　　Spectrally similar dyesCy5, Alexa Fluor® 647, DyLight® 649,

　　Molecular weight1032.18

　　Storage conditions-20C

　　Shipping conditionsAmbient temperature

IRDye 650 Maleimide

IR 650 Maleimide (IRDye® 650 Maleimide analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. IR 650 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

IRDye 650 Carboxylic acid

IR 650 Carboxylic acid (IRDye® 650 Carboxylic acid analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. IR 650 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

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本文由QiyueLh整理，2022年6月1日