IRDye 800CW NHS酯,IRDye 800CW NHS Ester
IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.
Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.
NHS Ester reacts with a primary aliphatic amine, such as lysine.
Dye label is attached through amide bond.
Dye Structure
Properties
Chemical Formula: C50H54N3Na3O17S4
Molecular Weight: 1166.20 g/mol
Exact Mass: 1165.20
IRDye 800CW 马来酰亚胺,IRDye 800CW Maleimide
IRDye 800CW Maleimide provides the functionality for labeling molecules that contain free sulfhydryl (–SH) groups, such as cysteine residues in proteins, with IRDye 800CW. The reactive group of the IRDye 800CW Maleimide allows conjugation reactions to be performed very efficiently at physiological pH.
Maleimide reacting with a thiol group.
Dye Structure
Properties
Chemical Formula: C52H57N4Na3O16S4
Molecular Weight: 1191.25 g/mol
Exact Mass: 1190.23
IRDye 800CW羧酸盐,IRDye 800CW Carboxylate
Assays that use IRDye 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.
Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.
Note: The carboxylate dye has no reactive group and cannot be used for labeling.
Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly and was completely cleared after 48 h. Originally published in Kovar, J., et al. (2007) Anal Biochem 367:1-12.
How is IRDye 800CW Carboxylate Used?
In vitro Cell-based Assays
As a control for cell-based assays that monitor binding of a dye-labeled agent.
Validation of optical agents for in vivo administration
Evaluation of binding specificity
In vivo Imaging
For evaluation of behavior and clearance of the dye itself.
Timing of dye clearance from the animal's body
Retention of dye in certain organs or sites (e.g., liver or kidneys)
Labeling Reaction Reference
As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.
Residual unreacted dye may cause:
Artificially high values when dye/protein (D/P) ratio is calculated
Increased background fluorescence in biological assays
Dye Structure
Properties
Chemical Formula: C46H50N2Na4O15S4
Molecular Weight: 1091.10 g/mol
Exact Mass: 1090.17
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本文由QiyueLh整理，2022年6月1日
