组蛋白1簇H4a(HIST1H4A)重组蛋白

组蛋白1簇H4a(HIST1H4A)重组蛋白

[ PROPERTIES ]

Residues: Ser2~Gly103 (Accession # P62806),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Nucleus. Chromosome.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 11.2

Predicted Molecular Mass: 12.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: Full length bovine HIST1H4A is identical in sequence to mouse HIST1H4Aand rat HIST1H4A. 100% cross-reactivity of HIST1H4A was observedamongbovine, mouse and rat.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget

protein. The loss rate was determined by accelerated thermal degradationtest,

that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF - SGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLARRGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYGFGG

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