CD2关联蛋白(CD2AP)重组蛋白

CD2关联蛋白(CD2AP)重组蛋白

[ PROPERTIES ]

Residues: Thr149~Glu429 (Accession # Q9Y5K6),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cytoplasm, cytoskeleton.

Cell projection, ruffle.

Purity: >95%

Endotoxin Level:<1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.8

Predicted Molecular Mass: 34.5kDa

Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducingconditions.Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget

protein. The loss rate was determined by accelerated thermal degradationtest,

that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TL NNKLGLFPSNFVKELEVTDDGETHEAQDDS ETVLAGPTSP IPSLGNVSET ASGSVTQPKK IRGIGFGDIF KEGSVKLRTRTSSSETEEKK PEKPLILQSL GPKTQSVEIT KTDTEGKIKA KEYCRTLFAY EGTNEDELTFKEGEIIHLIS KETGEAGWWR GELNGKEGVF PDNFAVQINE LDKDFPKPKK PPPPAKAPAPKPELIAAEKK YFSLKPEEKD EKSTLEQKPS KPAAPQVPPK KPTPPTKASN LLRSSGTVYPKRPEKPVPPP PPIAKINGE

[ REFERENCES ]

1.Kirsch K.H., et al. (2001) J. Biol. Chem. 276:4957-4963.

2. Kim J.M., et al. (2003) Science 300:1298-1300.

3. Monzo P., et al. (2005) Mol. Biol. Cell 16:2891-2902.

4. Konishi H., et al. (2006) J. Biol. Chem. 281:28919-28931.

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