70kDa热休克蛋白14(HSPA14)重组蛋白

70kDa热休克蛋白14(HSPA14)重组蛋白

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Met1~Ser509 (Accession # Q0VDF9),

with N-terminal His-Tag.

Host: E. coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm, cytosol.

Purity: Purity: Purity: Purity: >95%

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.8

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 56.0kDa

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG S -MAAIGVH LGC TSACVAVYKD GRAGVVANDA GDRVTPAVVA

YSENEEIVGL AAKQSRIRNI SNTVMKVKQI LGRSSSDPQA QKYIAESKCL VIEKNGKLRY

EIDTGEETKF VNPEDVARLI FSKMKETAHS VLGSDANDVV ITVPFDFGEK QKNALGEAAR

AAGFNVLRLI HEPSAALLAY GIGQDSPTGK SNILVFKLGG TSLSLSVMEV NSGIYRVLST

NTDDNIGGAH FTETLAQYLA SEFQRSFKHD VRGNARAMMK LTNSAEVAKH SLSTLGSANC

FLDSLYEGQD FDCNVSRARF ELLCSPLFNK CIEAIRGLLD QNGFTADDIN KVVLCGGSSR

IPKLQQLIKD LFPAVELLNS IPPDEVIPIG AAIEAGILIG KENLLVEDSL MIECSARDIL

VKGVDESGAS RFTVLFPSGT PLPARRQHTL QAPGSISSVC LELYESDGKN SAKEETKFAQ

VVLQDLDKKE NGLRDILAVLTMKRDGSLHV TCTDQETGKC EAISIEIAS

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