低密度脂蛋白受体相关蛋白5(LRP5)重组蛋白

低密度脂蛋白受体相关蛋白5(LRP5)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Asp769~Leu1016

Tags: N-terminal His-Tag

Tissue Specificity: Liver, Aorta. Subcellular Location: Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 31.9kDa

Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatethe

protein at 37

oC for 48h, and no obvious degradation and precipitationwereobserved.The loss rate is less than 5% within the expiration dateunderappropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE

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