碳酸酐酶Ⅶ(CA7)重组蛋白

碳酸酐酶Ⅶ(CA7)重组蛋白

[ DESCRIPTION ]

Protein Names: Carbonic Anhydrase VII

Synonyms: CA7, Car7

Species: Mouse

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Cytoplasm.

[ PROPERTIES ]

Residues: Ser58~Pro217 (Accession # Q9ERQ8), with N-terminal His-Tag.

Grade & Purity: >95%, 19 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

 Endotoxin Level: <1.0 EU per 1µg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 19.4 kDa

Predicted isoelectric point: 6.5

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious

degradation and precipitation were observed. (Referring from China Biological Products Standard, which was

calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-SLS ITNNGHSVQV DFNDSDDRTV VSGGPLEGPY RLKQLHFHWG KKRDMGSEHT VDGKSFPSEL

HLVHWNAKKY STFGEAAAAP DGLAVVGVFL ETGDEHPSMN RLTDALYMVR FKDTKAQFSC FNPKCLLPTS RHYWTYPGSL

TTPPLSESVT WIVLREP 

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