晶状体蛋白βB1(CRYbB1)重组蛋白

晶状体蛋白βB1(CRYbB1)重组蛋白

[ PROPERTIES ]

Residues: Lys6~Val186

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q9WVJ5

Host: E. coli

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 49.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget

protein. The loss rate was determined by accelerated thermal degradationtest,

that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

KASAT TAVNPGPDGK GKGAPSTGPA PAPGPTPVPA SVPRPAAKVGDLPPGSYRLI

VFEQENFQGR RVEFSGECLN LGDRGFDRVR SLIVVSGPWV AFEQSAFRGE MFVLEKGEYPRWDTWTSSYR SDRLMSFRPI RMDSQEHKIC LFEGANFKGN TMEIQEDDVP SLWVYGFCDRVGSITV

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