烯醇化酶(ENO3)重组蛋白

烯醇化酶(ENO3)重组蛋白

[ PROPERTIES ]

Residues: Met1~Lys391 (Accession # P13929),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.4

Predicted Molecular Mass: 46.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget

protein. The loss rate was determined by accelerated thermal degradationtest,

that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MAMQKIFARE ILDSRGNPTVEVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK AKFGANAILGVSLAVCKAGAAEKGVPLYRH IADLAGNPDL ILPVPAFNVI NGGSHAGNKL AMQEFMILPV GASSFKEAMRIGAEVYHHLK GVIKAKYGKD ATNVGDEGGF APNILENNEA LELLKTAIQA AGYPDKVVIGMDVAASEFYR NGKYDLDFKS PDDPARHITG EKLGELYKSF IKNYPVVSIE DPFDQDDWATWTSFLSGVNI QIVGDDLTVT NPKRIAQAVE KKACNCLLLK VNQIGSVTES IQACKLAQSNGWGVMVSHRS GETEDTFIAD LVVGLCTGQI KTGAPCRSER LAKYNQLMRI EEALGDKAIFAGRKFRNPKA K

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