中间α-球蛋白抑制因子H5(ITIH5)重组蛋白

中间α-球蛋白抑制因子H5(ITIH5)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Inter Alpha-Globulin Inhibitor H5

Synonyms: Synonyms: Synonyms: Synonyms: ITIH5, KIAA1953, PP14776, UNQ311

/PRO354

Species: Species: Species: Species: Human

Size: 50µg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Gln251~Asn508 (Accession # Q86UX2),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 33kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 30.5kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.9

Human ITIH5

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- QNGILGDFII RYDVNREQSI GDIQVLNGYF VHYFAPKDLP

PLPKNVVFVL DSSASMVGTK LRQTKDALFT ILHDLRPQDR FSIIGFSNRI KVWKDHLISV

TPDSIRDGKV YIHHMSPTGG TDINGALQRA IRLLNKYVAH SGIGDRSVSL IVFLTDGKPT

VGETHTLKIL NNTREAARGQ VCIFTIGIGN DVDFRLLEKL SLENCGLTRR VHEEEDAGSQ

LIGFYDEIRT PLLSDIRIDY PPSSVVQATK TLFPNYFN

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Dahlman I., et al. (2012). Arch. Physiol. Biochem. 118:84-91

2. Anveden A., et al. (2012) Obesity (Silver Spring) 20:708-714.

3. Rose J.E., et al. (2010) Mol. Med. 16:247-253.

4. Karasik D., et al. (2010) J. Bone Miner. Res. 25:1555-1563.

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