翻译调节肿瘤蛋白1(TPT1)重组蛋白

翻译调节肿瘤蛋白1(TPT1)重组蛋白

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Met1~Cys172 (Accession # P13693),

with N-terminal His-Tag.

Host: E. coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Purity: Purity: Purity: Purity: >95%

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.2

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 21.1kDa

Accurate Accurate Accurate Accurate Molecular Molecular Molecular Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- MIIYRD LISH DEMFSDIYKI REIADG LC LE VEGKMVSRTE

GNIDDSLIGG NASAEGPEGE GTESTVITGV DIVMNHHLQE TSFTKEAYKK YIKDYMKSIK

GKLEEQRPER VKPFMTGAAE QIKHILANFK NYQFFIGENM NPDGMVALLD YREDGVTPYM

IFFKDGLEME KC

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1.Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.

2. Olsen J.V., et al. (2010) Sci. Signal. 3:RA3-RA3.

3. Dong X., et al. (2009) J. Biol. Chem. 284:23754-23764.

4. Dephoure N., et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767.

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