蛋白磷酸酶3调节因子亚基1(PPP3R1)重组蛋白
产品详情:
- 中文名称:
- 蛋白磷酸酶3调节因子亚基1(PPP3R1)重组蛋白
- 英文名称:
- Recombinant Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1)
- 保存条件:
- 避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月
- 纯度规格:
- > 95%
- 产品类别:
- 重组蛋白
- 货号::
- BES21903RP
蛋白磷酸酶3调节因子亚基1(PPP3R1)重组蛋白
[ PROPERTIES ]
Residues: Gly2~Val170
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P63098
Host: E. coli
Subcellular Location: Cytoplasm. Cell membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 4.6
Predicted Molecular Mass: 49.2kDa
Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducingconditions.Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQQNPLVQRVIDIFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNGELFQVLKMMVGNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
公司简介
成立日期 | (5年) |
注册资本 | 100万 |
员工人数 | 10-50人 |
年营业额 | ¥ 100万以内 |
经营模式 | 试剂,服务 |
主营行业 | 生化试剂,抗体,蛋白组学,分子生物学,细胞生物学 |
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