氨基乙二酸转氨酶(AADAT)重组蛋白

氨基乙二酸转氨酶(AADAT)重组蛋白

[ PROPERTIES ]

Residues: Asn202~Ser425

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8N5Z0

Host: E. coli

Subcellular Location: Mitochondrion. Mitochondrial

Matrix.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 29.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget

protein. The loss rate was determined by accelerated thermal degradationtest,

that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

NPTGNSLTS ERKKEIYELA RKYDFLIIED DPYYFLQFNK FRVPTFLSMD VDGRVIRADSFSKIISSGLR IGFLTGPKPL IERVILHIQV STLHPSTFNQ LMISQLLHEWGEEGFMAHVDRVIDFYSNQK DAILAAADKW LTGLAEWHVP AAGMFLWIKV KGINDVKELI EEKAVKMGVLMLPGNAFYVD SSAPSPYLRA SFSSASPEQM DVAFQVLAQL IKESL

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