血小板反应蛋白4(THBS4)重组蛋白

血小板反应蛋白4(THBS4)重组蛋白

[ PROPERTIES ]

Residues: Pro644~Cys925 (Accession # P35443),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 63.4kDa

Accurate Molecular Mass: 61kDa as determined by SDS-PAGE reducingconditions.Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetarget

protein. The loss rate was determined by accelerated thermal degradationtest,

that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PTVINSA QLDTDKDGIGDECDDDDDND GIPDLVPPGP DNCRLVPNPA QEDSNSDGVG DICESDFDQDQVIDRIDVCPENAEVTLTDF RAYQTVVLDP EGDAQIDPNW VVLNQGMEIV QTMNSDPGLA VGYTAFNGVDFEGTFHVNTQ TDDDYAGFIF GYQDSSSFYV VMWKQTEQTY WQATPFRAVA EPGIQLKAVKSKTGPGEH LR NS LWHTGDTS DQV R LLWK DS RNVGWK DKVS YRW FLQHRPQVGYIRVRFYE GSELVADSGV TIDTTMRGGR LGVFC

[ REFERENCES ]

1.Lawler J., et al. (1993) J. Mol. Evol. 36:509-516.

2. Lawler J., et al. (1995) J. Biol. Chem. 270:2809-2814.

3. Zhou X., et al. (2004) Clin. Sci. 106:495-500.

4. Wessel J., et al. (2004) Am. Heart J. 147:905-909.

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