细胞色素P450家族成员1A1(CYP1A1)重组蛋白

细胞色素P450家族成员1A1(CYP1A1)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Met1~Ala250

Tags: N-terminal His-Tag

Tissue Specificity: Lung Cancer. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membraneprotein. Microsome membrane. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 31.2kDa

Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducingconditions.Phenomenon explanation:

The possible reasons that the actual band size differs fromthe predictedareasfollows:Splice variants: Alternative splicing may create different sized proteins fromthe samegene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37

oC for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE

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