重组人缺氧诱导因子 1 α

中文名称

重组人缺氧诱导因子 1 α

英文名字

Recombinant Human Hypoxia-Inducible Factor-1 Alpha

供应商

ProSpec

产品货号

PRO-477

产品报价

￥800/10ug   ￥2080/50ug   ￥28980/1mg

产品说明书

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产品新闻

背景资料

Hypoxia-inducible factor-1 （HIF-1）， identified as one of the transcription factors， has been found to play an essential role in cellular and systemic oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1? subunit and one of three subunits （Hif-1?， Hif-2 （or Hif-3））. The activation of Hif-1 （is closely associated with a variety of tumors and oncogenic pathways. Hif-1 （consists of DNA binding domain （DBD domain）， Dimerization domain and C-terminal regulatory domains， including two transactivation domains （TAD）， an oxygen-dependent degradation （ODD） domain， and inhibitory domains. Under hypoxic conditions HIF1A activates the transcription of more than 40 genes， including， erythropoietin， glucose transporters， glycolytic enzymes， VEGF， and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1A also plays a crucial role in embryonic vascularization， tumor angiogenesis and pathophysiology of ischemic disease. It binds to core DNA sequence 5’-[AG]CGTG-3’ within the hypoxia response element （HRE） of target gene promoters. Activation involves recruitment of transcriptional coactivators such as CREBPB and EP300. Its activity is improved by interaction with both， NCOA1 or NCOA2. Interaction with redox regulatory protein APEX appears to activate CTAD and potentiates activation by NCOA1 and CREBBP. The induction is under reduced oxygen tension. HIF1A is also induced by a variety of receptor-mediated factors such as growth factors， cytokines， and circulatory factors for example PDGF， EGF， FGF-2， IGF-2， TGF-1 beta， HGF， TNF alpha， IL-1 beta， angiotensin-2 and thrombin. Nevertheless， this induction is less intense than that stimulated by hypoxia.

序列

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来源宿主

大肠杆菌中重组表达

溶解建议

Sterile filtered liquid formulation. The HIF1A recombinant Human solution is formulated 20mM Tris-HCl pH-7.5 and 1mM DTT.

保存建议

MOP1 Recombinant Human although stable at 4℃ for 30 days， should be stored desiccated below -20℃ for periods greater than 30 days. Please avoid freeze-thaw cycles.

其他

ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。 领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。