重组结核分枝杆菌早期分泌抗原

中文名称

【EAST6】重组结核分枝杆菌早期分泌抗原

英文名字

Recombinant Early Secretory Target Mycobacterium Tuberculosis

供应商

ProSpec

产品货号

PRO-291

产品报价

￥800/2ug   ￥2080/10ug   ￥83720/1mg

产品说明书

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产品新闻

背景资料

Mycobacterium antigen ESAT-6 has been isolated from low molecular weight fractions of the shot-term-culture filtrate （ST-CF） and it can easily be detected in tuberculosis patients.
The export of ESAT-6 which is a potent T-cell antigen， and related proteins requires a dedicated secretory apparatus that is encoded by a group of genes， several of which also code for proteins that are recognized strongly by T cells. The ESAT-6 systems can consequently be considered as immunogenicity islands and there is mounting evidence that the equivalent genes are subject to selective pressure imposed by the immune system of the host.
This antigen includes many epitopes detectable in the serum of most patients with tuberculosis （more than 90%）. By the attempts to obtain the vaccine on the basis of ESAT-6 it was demonstrated that the optimization of adjuvant is very important when using the combination of dioctadecylammonium bromide and monophosphoryllipide.
Recently it was shown that ESAT-6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. The main topic in ESAT-6 using is in antibody production and in test-systems for tuberculosis elaboration.

序列

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来源宿主

大肠杆菌中重组表达

溶解建议

Sterile Filtered White lyophilized （freeze-dried） powder. The protein （1mg/ml） was lyophilized after from a sterile solution containing 50mM Tris-HCl， pH 8.0， 1mM EDTA and 1mM DTT. It is recommended to reconstitute the lyophilized ESAT-6 in sterile 18MΩ-cm H2O not less than 100ug/ml， which can then be further diluted to other aqueous solutions.

保存建议

Lyophilized ESAT-6 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution ESAT-6 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.

其他

ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。 领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。