微粒体甘油三酸酯转运蛋白(MTTP)重组蛋白

微粒体甘油三酸酯转运蛋白(MTTP)重组蛋白

[ PROPERTIES ]

Residues: Leu28~Gly459

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O08601

Host: E. coli

Subcellular Location: Endoplasmic reticulum.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 47.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

LNN ERLYKLTYST EVFLDGGKGK PQDSVGYKIS SDVDVVLLWR NPDGDDDQVI

QVTITAVNVE NAGQQRGEKS IFQGKSTPKI IGKDNLEALQ RPMLLHLVRG KVKEFYSYEN

EPVGIENLKR GLASLFQMQL SSGTTNEVDI SGDCKVTYQA QQDKVVKIKA LDTCKIERSG

FTTANQVLGV SSKATSVTTY KIEDSFVTAV LAEETRAFAL NFQQTIAGKI VSKQKLELKT

TEAGPRMIPG KQVAGVIKAV DSKYKAIPIV GQVLERVCKG CPSLAEHWKS IRKNLEPENL

SKAEAVQSFL AFIQHLRTSR REEILQILKA EKKEVLPQLV DAVTSAQTPD SLEAILDFLD

FKSDSSIILQ ERFLYACGFA THPDEELLRA LLSKFKGSFA SNDIRESVMI IIGALVRKLC

QNEGCKLKAV VEAKKLILG

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