肘臀蛋白(CUBN)重组蛋白

肘臀蛋白(CUBN)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Cys3157~Met3274

Tags: N-terminal His-Tag

Homology: Mouse 70%, rat 70%

Tissue Specificity: Placenta, intestine, kidney. Subcellular Location: Endosome membrane; Peripheral membrane protein. Lysosome membrane. Purity: >95%

Endotoxin Level:<1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 14.4kDa

Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PA

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