Ⅸ型胶原α1(COL9a1)重组蛋白

Ⅸ型胶原α1(COL9a1)重组蛋白

[ PROPERTIES ]

Residues: Phe24~Leu268

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P20850

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.1

Predicted Molecular Mass: 31.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

FFACSFL GPCVSATPKR RARFPANSVS HGGSELCPKI RIGQDDLPGF DLISQFQIEK

AASRRTIQRV VGSTALQVAY KLGSNVDFRI PTRHLYPSGL PEEYSFLTTF RMTGSTLEKQ

WNIWQIQDSS GREQVGVKIN GQTKSVAFSY KGLDGSLQTA AFLNLQSLFD SRWHKLMIGV

ERTRATLFID CIQIDSLPIK PRAQIDADGF AVLGKLVDNP QVSVPFELQW MLIHCDPLRP

RRETCHEL

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