核因子κB受体激活因子(RANk)重组蛋白

核因子κB受体激活因子(RANk)重组蛋白

[ PROPERTIES ]

Residues: Val330~Lys615 (Accession # Q9Y6Q6),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 33.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its

sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- V SKTEIEEDSF RQMPTEDEYM

DRPSQPTDQL LFLTEPGSKS TPPFSEPLEV GENDSLSQCF TGTQSTVGSE SCNCTEPLCR

TDWTPMSSE N YLQKEVDSGH CPHWAASPSP NWA DV CTGCR NPPGE DCEP L

VGSPK RGP LP QCAYGMG LP P EEEAS RTEA R DQPE DGA DGR LPS SA RAGAG

SGSSPGGQSP ASGNVTGNSN STFISSGQVM NFKGDIIVVY VSQTSQEGAA AAAEPMGRPV

QEETLARRDS FAGNGPRFPD PCGGPEGLRE PEKASRPVQE QGGAK

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