脂肪酸结合蛋白5(FABP5)重组蛋白

脂肪酸结合蛋白5(FABP5)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Fatty Acid Binding Protein 5, Epidermal

Gene Names: FABP5

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: High specificity for fatty acids. Highest affinity for C18 chain length.

Decreasing the chain length or introducing double bonds reduces the affinity. May

be involved in keratinocyte differentiation.

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm.

Tissue Specificity: Specificity: Specificity: Specificity: Keratinocytes; highly expressed in psoriatic skin.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Ala2~Glu135 (Accession # Q01469), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 16.3 kDa as determined by SDS-PAGE reducing

conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 16.3 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will

increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGS-ATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL

KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI

YEKVE

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Madsen P.S., et al. (1992) J. Invest. Dermatol. 99:299-305.

2. Gutierrez-Gonzalez L.H., et al. (2002) Biochem. J. 364:725-737.

3. Hohoff C., et al. (1999) Biochemistry 38:12229-12239.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！