Smad同源物4(Smad4)重组蛋白

Smad同源物4(Smad4)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Trp323~Asp552

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Muscle. Subcellular Location: Cytoplasm. Nucleus. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 28.7kDa

Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions. 

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE

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