成肌分化蛋白(MyoD)重组蛋白

成肌分化蛋白(MyoD)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Met1~Leu320

Tags: N-terminal His Tag

Subcellular Location: Nucleus

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% SKL, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 38.2kDa

Accurate Molecular Mass: 50kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved

to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not

vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8ºC for one month.

 Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined

by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no

obvious degradation and precipitation were observed. The loss rate is less than 5% within the

expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure. Gene Sequencing (Extract)

Figure. SDS-PAGE

[ IMPORTANT NOTE ]

The kit is designed for in vitro and research use only, we will not be responsible for any issue if

the kit was used in clinical diagnostic or any other procedures.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！