10kDa热休克蛋白1(HSP10)重组蛋白

10kDa热休克蛋白1(HSP10)重组蛋白

[ PROPERTIES ]

Residues: Ala2~Asp102 (Accession # P61604),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Mitochondrion matrix. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 8.0

Predicted Molecular Mass: 12.3kDa

Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF -AGQAFRKFL PLFDRVLVE R SAAETVTKGG IM LPEKSQGK

VLQATVVAVG SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY

VD

[ REFERENCES ]

1.Monzini N., et al. (1994) Biochim. Biophys. Acta 1218:478-480.

2. Chen J.J., et al. (1994) Biochim. Biophys. Acta 1219:189-190.

3. Hansen J.J., et al. (2003) Hum. Genet. 112:71-77.

4. Cavanagh A.C., Morton H. (1994) Eur. J. Biochem. 222:551-560.

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