糖原磷酸化酶L(PYGL)重组蛋白

糖原磷酸化酶L(PYGL)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Glycogen Phosphorylase, Liver

Synonyms Synonyms Synonyms Synonyms: PYGL, GPLL

Species: Species: Species: Species: Human

Size: 100µg

Source: Escherichia coli-derived

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Asn633~Glu820 (Accession # P06737), with N-terminal His-Tag.

Grade & Purity: Purity: Purity: Purity: >97%, 22.6 kDa as determined by SDS-PAGE reducing conditions.

Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 22.6 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.2

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months . Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious

degradation and precipitation were observed. (referring from China Biological Products Standard, which was

calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES]

The target protein is fused with one N-terminal His-tag, its sequence is listed below.

NDPMVGSK LKVIFLENYR VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM

AEEAGEENLF IFGMRIDDVAALDKKGYEAK EYYEALPELK LVIDQIDNGF FSPKQPDLFK DIINMLFYHD

RFKVFADYEA YVKCQDKVSQ LYMNPKAWNT MVLKNIAASG KFSSDRTIKE

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Livanova NB., et al. (2002) Biochemistry (Moscow) 67 (10): 1089–1998. 2. Palm D., et al. (1990) Biochemistry 29 (5): 1099–1107.

3. Browner MF., et al. (2005) Trends in Biochemical Science 17 (2): 66–71.

4. Johnson LN., et al. (1992) FASEB Journal 6 (6): 2274–82. 5. Newgard CB., et al. (1989) Critical Reviews Biochemistry and Molecular Biology 24 (1): 69–99.

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