心肌营养素1(CT1)重组蛋白

心肌营养素1(CT1)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Cardiotrophin 1

Synonyms: Synonyms: Synonyms: Synonyms: CT1

Size: 100 µg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Ala201 (Accession # Q16619), with one N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >95%, 22.7 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 22.7 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.7

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS, PH7.2-PH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months .

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious

degradation and precipitation were observed. (Referring from China Biological Products Standard, which was

calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with one N-terminal His-tag. Their sequence is listed below.

MGHHHHHHSGSEF MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG

LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP

RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Pennica D., et al. (1996) Cytokine. 8(3):183-9.

2. Jougasaki M. (2010) Adv Clin Chem. 52:41-76.

3. Latchman DS. (2000) Pharmacol Ther. 85(1):29-37.

4. Railson JE., et al. (2002) Cytokine. 17(5):243-53.

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